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[Utilization of an ELISA technic for the quantification of antipoliovirus antibodies in human sera]
A double antibody enzyme linked immunosorbent assay (ELISA) was elaborated for detection of poliovirus antibodies in human sera. The IgG to be titrated were immunoabsorbed by capture on the solid phase. The antigens used were obtained from vero cell cultures (green Monkey Kidney Cells). The reaction was followed by adding rabbit antipoliovirus serum, then sheep Fab fragment prepared against rabbit IgG and labelled with horse radish peroxidase. Ortho-tolidine was used as the chromogen substrate to reveal the reaction. This enabled a first reading with the naked eye. This technique allows to keep a better track of the poliomyelitis immunization.
A double antibody enzyme linked immunosorbent assay (ELISA) was elaborated for detection of poliovirus antibodies in human sera. The IgG to be titrated were immunoabsorbed by capture on the solid phase. The antigens used were obtained from vero cell cultures (green Monkey Kidney Cells). The reaction was followed by adding rabbit antipoliovirus serum, then sheep Fab fragment prepared against rabbit IgG and labelled with horse radish peroxidase. Ortho-tolidine was used as the chromogen substrate to reveal the reaction. This enabled a first reading with the naked eye. This technique allows to keep a better track of the poliomyelitis immunization.