Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Heparin-binding angiogenesis factors: detection by immunological methods.

M G Wadzinski, J Folkman, J Sasse

    Clinical Physiology and Biochemistry
    |January 1, 1987
    PubMed
    Summary

    This study details immunological methods for detecting basic fibroblast growth factor (FGF). Researchers confirmed a human hepatoma cell line produces a growth factor similar to basic FGF found in the brain and pituitary.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Zoonotic origin and transmission of Middle East respiratory syndrome coronavirus in the UAE.

    Zoonoses and public health·2017
    Same author

    Protein analysis by SDS-PAGE and detection by Coomassie blue or silver staining.

    Current protocols in pharmacology·2011
    Same author

    Disseminated multiorgan MDR-TB resistant to virtually all first-line drugs.

    European respiratory review : an official journal of the European Respiratory Society·2010
    Same author

    [Specific aspects of treatment for women with bipolar affliction].

    Der Nervenarzt·2009
    Same author

    Matrigel cytometry: a novel method for quantifying angiogenesis in vivo.

    Journal of immunological methods·2008
    Same author

    Xenogenic oogenesis of chicken (Gallus domesticus) female primordial germ cells in germline chimeric quail (Coturnix japonica) ovary.

    Animal reproduction science·2007

    Area of Science:

    • Immunology
    • Molecular Biology
    • Biochemistry

    Background:

    • Basic fibroblast growth factor (FGF) plays crucial roles in cell growth, differentiation, and tissue repair.
    • Accurate detection methods are essential for understanding FGF's biological functions and its involvement in diseases.

    Purpose of the Study:

    • To describe and validate immunological techniques for detecting basic FGF.
    • To investigate the synthesis of basic FGF by a human hepatoma cell line.

    Main Methods:

    • Development of polyclonal antibodies against synthetic peptides of basic FGF.
    • Application of five immunological detection methods: ELISA, immunoblot, Western blot, immunoprecipitation, and radioimmunoassay.
    • Analysis of basic FGF expression in a human hepatoma cell line.

    Related Experiment Videos

    Main Results:

    • Successfully raised polyclonal antibodies specific to basic FGF.
    • Demonstrated the utility of the five immunological methods for basic FGF detection.
    • Confirmed that the human hepatoma cell line synthesizes a growth factor structurally related to brain and pituitary basic FGF.

    Conclusions:

    • Immunological methods provide reliable means for basic FGF detection.
    • Human hepatoma cells express a basic FGF-related growth factor, suggesting its potential role in hepatoma development.