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Published on: August 2, 2016
Fabrication of a Cell Fixation Device for Robotic Cell Microinjection
Yu Xie1, Yunlei Zhou2, Wenming Xi3
1School of Aerospace Engineering, Xiamen University, Xiamen 361005, China. xieyu@xmu.edu.cn.
Abstract:
Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surface to control the contact force between cells and the material. Data show that when the injection velocity exceeds 1.5 mm/s, microinjection success rate is over 80%. The maximum value of the adhesion force between the PDMS plate and the cell is 0.0138 N, and the need can be met in practical use of the robotic microinjection.
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