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Related Concept Videos

Archival Research01:40

Archival Research

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Some researchers gain access to large amounts of data without interacting with a single research participant. Instead, they use existing records to answer various research questions. This type of research approach is known as archival research. Archival research relies on looking at past records or data sets to look for interesting patterns or relationships. For example, a researcher might access the academic records of all individuals who enrolled in college within the past ten years and...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Sampling materials are classified into three main types: solid, liquid, and gas.
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The gastrointestinal tract, responsible for the digestion and absorption of nutrients, is safeguarded by the intestinal barrier, which consists of secretory, physical, and immune components. At the forefront is the secretory barrier, composed of essential elements such as mucus, gut microbiota, and defense proteins. They collaborate to break down food particles, facilitate nutrient absorption, and maintain optimal gut health. These secretory components ensure the smooth functioning of the...
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Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
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Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
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Murine Fecal Isolation and Microbiota Transplantation
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Evaluating gut microbiota profiles from archived fecal samples.

Trine B Rounge1, Roger Meisal2, Jan Inge Nordby3

  • 1Department of Research, Cancer Registry of Norway, Oslo, Norway. trine.rounge@kreftregisteret.no.

BMC Gastroenterology
|November 10, 2018
PubMed
Summary

Archived fecal immunochemical test (iFOBT) samples are suitable for gut microbiota studies, showing no contamination or evaporation. Long-term archived samples require careful handling for accurate microbiome analysis.

Keywords:
Archived samplesDiversityFecal immunochemical testsFecal samplesMicrobiotaStorage

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Area of Science:

  • Microbiome research
  • Colorectal cancer studies
  • Biomarker discovery

Background:

  • Gut microbiota alterations are linked to colorectal cancer.
  • Archived fecal samples offer potential for longitudinal studies in carcinogenesis and biomarker discovery.
  • Assessing gut microbiota quality and stability in archived samples is crucial before large-scale studies.

Purpose of the Study:

  • Evaluate cross-contamination in fecal occult blood tests (iFOBT).
  • Assess sample evaporation and temperature stability of stored iFOBT samples.
  • Compare gut microbiota diversity and composition across fresh, iFOBT, and archived fecal samples.

Main Methods:

  • Microbiota profiling using 16S rDNA gene V3-V4 region sequencing.
  • Quantitative PCR (qPCR) to detect cross-contamination.
  • Evaluation of evaporation and temperature stability during sample handling and storage.

Main Results:

  • iFOBT analysis showed no detectable cross-sample contamination or evaporation.
  • Gut microbiome stability was confirmed at room temperature.
  • Microbial profiles were obtained from 100% of fresh, 96% of iFOBT, and 81% of long-term archived samples.
  • Microbiota diversity and composition were comparable between fresh and iFOBT samples, but differed significantly in long-term archived samples.

Conclusions:

  • Archived fecal samples from iFOBT are feasible for microbiota studies, offering valuable diagnostic and follow-up data.
  • These samples facilitate microbial biomarker discovery and longitudinal observational studies.
  • Caution is advised when profiling the gut microbiota of long-term archived fecal samples.