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Updated: Feb 2, 2026

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
Published on: October 2, 2016
Rapid quantification of trace chloramphenicol in honey under ambient conditions using direct analysis via real-time
Xiu Qin Li1, Hong Mei Li1, Sen Xu2
1Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, PR China.
Abstract:
In this paper, we employed a direct analysis in real time (DART) ion source coupled to QTRAP mass spectrometry for the analysis of chloramphenicol (CAP) in honey. The accuracy of the DART-MS/MS method for the analysis of CAP in honey was evaluated by comparison with data generated by a validated HPLC-MS/MS method. The sample preparation procedure was optimized to obtain sensitive and accurate determination of trace CAP residue in honey at concentrations less than 1.0 µg/kg. The DART-MS/MS method offers faster analysis time, lower cost per analysis, and reduced matrix effects and simplicity compared to HPLC-MS/MS method. Fifty-two honey samples collected from a Chinese market were analyzed using two methods. The results of the two methods are in good agreement, suggesting DART-MS/MS as a potential technique for the direct detection of trace amounts of veterinary drugs in complex matrixes.
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