Related Experiment Video For Procollagen C-proteinase enhancer 1
Updated: Feb 2, 2026

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method
Published on: May 21, 2018
Synergistic effect of PCPE1 and sFRP2 on the processing of procollagens via BMP1
Qin Zhu1,2, Wei Guo1,2,3, Shengjie Zhang1,2
1Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, China.
Abstract:
Procollagen processing is essential for organ development and tissue functions. Both procollagen C-proteinase enhancer 1 (PCPE1) and secreted frizzled-related protein 2 (sFRP2) play vital roles in collagen formation via regulating the procollagen C-proteinase activity of bone morphogenetic protein 1 (BMP1). However, whether the two proteins exert a synergistic effect on BMP1 activity remains unclear. Here, simultaneous knockdown of sFRP2 and PCPE1 led to less collagen formation in mouse embryonic fibroblasts and dorsalized phenotypes in zebrafish embryos. Further studies revealed a direct interaction between the Frizzled domain of sFRP2 and the complement/Uegf/BMP-1 domain of PCPE1, which enhances the cleavage activity of BMP1 on procollagen. These results suggest that double silencing of sFRP2 and PCPE1 may provide a strategy for treating fibrosis diseases caused by collagen deposition.
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