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Updated: Feb 2, 2026

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Published on: January 18, 2020
Analytical Quality by Design-based development and validation of ultra pressure liquid chromatography/MS/MS method
Ana Stajić1, Jelena Maksić2, Đoko Maksić3
1Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade, Serbia.
Aim:
An ultra pressure liquid chromatography (UPLC)/MS/MS method for vancomycin and teicoplanin determination in human plasma was developed in accordance with analytical quality by design (AQbD) concept and fully validated.
Materials & Methods:
Chromatographic separation was performed on ACQUITY UPLC C18 charge surface hybrid (CSH) column (2.1 mm × 50 mm, 1.7 μm particle size) in gradient mode and the mobile phase consisted of 0.1% formic acid in water and pure acetonitrile. The experimental design methodology was used for the definition of optimal chromatographic and protein precipitation conditions.
Results:
The linearity ranges were 0.05-10 μg ml-1 for vancomycin and 0.5-200 μg ml-1 for total teicoplanin. The relative standard deviations for precision estimation were below 15% and the accuracy was within 85-115% for all quality control levels.
Conclusion:
The method was utilized for glycopeptide antibiotics bioanalysis.
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