Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
2.1K
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

81.1K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
81.1K
Membrane Proteins01:30

Membrane Proteins

30.5K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
30.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular insights into the dual-glycoprotein surface layer of the oral bacterium Tannerella serpentiformis.

Journal of molecular graphics & modelling·2026
Same author

Navigating the Pre- and Post-AlphaFold Divide: CAPRI 8th Evaluation Meeting, February 12-14, Grenoble, FR.

Proteins·2025
Same author

Updates to the CASP Infrastructure in 2024.

Proteins·2025
Same author

Improved prediction of antibody and their complexes with clustered generative modelling ensembles.

Bioinformatics advances·2025
Same author

Biomolecular Interaction Prediction in the Pre- and Post-AlphaFold Era: The 8th CAPRI Evaluation.

Proteins·2025
Same author

Molecular determinants for recognition of serotonylated chromatin.

Nucleic acids research·2025

Related Experiment Video

Updated: Feb 2, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K

A Membrane Protein Complex Docking Benchmark.

Panagiotis I Koukos1, Inge Faro1, Charlotte W van Noort1

  • 1Bijvoet Center for Biomolecular Research, Faculty of Science-Chemistry, Utrecht University, Padualaan 8, Utrecht 3584CH, the Netherlands.

Journal of Molecular Biology
|November 12, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces the first membrane protein-protein docking benchmark with 37 diverse targets. It establishes HADDOCK

Keywords:
HADDOCKdockingmembrane proteinsprotein–protein complexesscoring

More Related Videos

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
08:12

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Published on: September 28, 2018

13.7K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.2K

Related Experiment Videos

Last Updated: Feb 2, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
08:12

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Published on: September 28, 2018

13.7K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.2K

Area of Science:

  • Structural biology
  • Computational biophysics
  • Biochemistry

Background:

  • Membrane protein-protein interactions are crucial for cellular functions.
  • Accurate prediction of these interactions is challenging due to the complexity of membrane environments.
  • Existing protein-protein docking tools often require specific adaptations for membrane proteins.

Purpose of the Study:

  • To create the first benchmark dataset for membrane protein-protein docking.
  • To evaluate the performance of the HADDOCK software for membrane protein complex prediction.
  • To provide a resource for developing and optimizing membrane-specific docking scoring functions.

Main Methods:

  • Compilation of 37 diverse membrane protein targets with available unbound structures.
  • Data cleaning and consistent numbering for standardized docking.
  • Evaluation of HADDOCK performance in two scenarios: interface-driven and ab initio docking.
  • Analysis of docking results and generation of decoy sets.

Main Results:

  • The benchmark dataset includes 37 diverse membrane protein targets.
  • HADDOCK demonstrated promising, albeit baseline, performance for membrane protein docking without specific optimization.
  • The study identified areas for improvement in docking algorithms for membrane systems.

Conclusions:

  • The developed benchmark is a valuable resource for the field of membrane protein-protein docking.
  • HADDOCK shows potential for docking membrane protein complexes, indicating a need for further optimization.
  • The freely available decoys and scripts will facilitate the development of specialized scoring functions.