Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Solubility of Ionic Compounds02:55

Solubility of Ionic Compounds

68.2K
Solubility is the measure of the maximum amount of solute that can be dissolved in a given quantity of solvent at a given temperature and pressure. Solubility is usually measured in molarity (M) or moles per liter (mol/L). A compound is termed soluble if it dissolves in water.
68.2K
Factors Affecting Solubility04:01

Factors Affecting Solubility

37.1K
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Chȃtelier’s principle. Consider the dissolution of silver iodide:
37.1K
Solubility Equilibria03:07

Solubility Equilibria

57.4K
Solubility equilibria are established when the dissolution and precipitation of a solute species occur at equal rates. These equilibria underlie many natural and technological processes, ranging from tooth decay to water purification. An understanding of the factors affecting compound solubility is, therefore, essential to the effective management of these processes. This section applies previously introduced equilibrium concepts and tools to systems involving dissolution and precipitation.
The...
57.4K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.8K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.8K
Physical Properties Affecting Solubility02:19

Physical Properties Affecting Solubility

26.5K
Solutions of Gases in Liquids
As for any solution, the solubility of a gas in a liquid is affected by the attractive intermolecular forces between solute and solvent species. Unlike solid and liquid solutes, however, there is no solute-solute intermolecular attraction to overcome when a gaseous solute dissolves in a liquid solvent since the atoms or molecules comprising a gas are far separated and experience negligible interactions. Consequently, solute-solvent interactions are the sole...
26.5K
Solubility03:00

Solubility

21.1K
Solution, Solubility, and Solubility Equilibrium
A solution is a homogeneous mixture composed of a solvent, the major component, and a solute, the minor component. The physical state of a solution—solid, liquid, or gas—is typically the same as that of the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
In a solution, the solute particles (molecules,...
21.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Affinity-Tuned Albumin Hitchhiking Extends the Bioorthogonal Capture Window in Pretargeting Radiotheranostics.

Advanced healthcare materials·2026
Same author

Epitope-Resolved Digital SERS Profiling of Structurally Dynamic Antigens via a Multi-Epitope Bispecific Antibody Framework.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Cell-Mimetic Antifouling Poly(ethylene glycol) Interface Enables Selective Capture and Viable Release of Circulating Tumor Cells.

The journal of physical chemistry. B·2026
Same author

Spatially Profiling Trace Cytokine Signatures From Microscopically Derived Skin Samples to Probe Skin Disease Inflammation.

Small methods·2026
Same author

Monkeypox (Mpox), a Resurging Global Public Health Concern: An Updated Outlook Through 2025.

Current issues in molecular biology·2026
Same author

Multifactor authentication in extracellular vesicle analysis: methods and approaches to address the heterogeneity problem.

Nature methods·2026
Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026
Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026
Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026
Same journal

Synergistic mode-field pre-expansion and geometric compression in hetero-structured microfibers for ultrasensitive glucose sensing.

Biosensors & bioelectronics·2026
Same journal

An amplification-free dual-readout biosensor integrating colorimetry and single-particle counting for ultrasensitive miRNA detection in esophageal cancer.

Biosensors & bioelectronics·2026
Same journal

An all-in-one microfluidic system via data-driven design for on-site genotyping of genetically modified foods.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: Feb 2, 2026

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans
09:00

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

Published on: May 2, 2018

7.0K

A SERS microfluidic platform for targeting multiple soluble immune checkpoints.

K Kamil Reza1, Abu Ali Ibn Sina1, Alain Wuethrich1

  • 1Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Corner College and Cooper Roads (Bldg 75), Brisbane, QLD 4072, Australia.

Biosensors & Bioelectronics
|November 12, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a novel SERS-microfluidics device for detecting immune checkpoint proteins. The platform offers a cost-effective and sensitive method for monitoring cancer immunotherapies using nano yeast single chain variable fragments (scFvs).

Keywords:
CancerImmune checkpointsMicrofluidic deviceSERSac-EHD

More Related Videos

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
10:13

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

11.8K
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

13.4K

Related Experiment Videos

Last Updated: Feb 2, 2026

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans
09:00

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

Published on: May 2, 2018

7.0K
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
10:13

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

11.8K
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
08:02

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Published on: November 7, 2013

13.4K

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Analytical Chemistry

Background:

  • Immune checkpoint blockade therapies are crucial for cancer treatment.
  • Monitoring therapeutic response via soluble immune checkpoint markers in body fluids is desirable but challenging.
  • Current detection methods using monoclonal antibodies are costly and time-consuming.

Purpose of the Study:

  • To develop an integrated surface-enhanced Raman scattering (SERS)-microfluidics device for sensitive and multiplexed detection of soluble immune checkpoint proteins.
  • To utilize nano yeast single chain variable fragments (scFvs) as a cost-effective alternative to monoclonal antibodies.
  • To overcome limitations of current detection methods for monitoring cancer immunotherapies.

Main Methods:

  • An integrated SERS-microfluidics platform was developed.
  • Nano yeast single chain variable fragments (scFvs) were employed as biorecognition elements.
  • Graphene oxide functionalized surfaces and alternating current electrohydrodynamics (ac-EHD) induced nanomixing were utilized.
  • SERS-based spectral encoding enabled multiplexed detection.

Main Results:

  • The device successfully detected soluble immune checkpoints PD-1, PD-L1, and LAG-3.
  • Detection limits as low as 100 fg/mL were achieved in human serum.
  • The platform demonstrated high specificity and minimized non-specific interactions.

Conclusions:

  • The developed SERS-microfluidics device offers a promising, low-cost, and sensitive approach for detecting immune checkpoint biomarkers.
  • This technology has the potential to revolutionize the monitoring of cancer immunotherapies.
  • Nano yeast scFvs provide a viable alternative to traditional monoclonal antibodies in diagnostic applications.