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The dento-gingival junction as seen with light microscopy and scanning electron microscopy.
1Department of Periodontics, Medical College of Georgia, School of Dentistry, Augusta 30912.
Scanning Microscopy
|June 1, 1988
Summary
This study reviews the dento-gingival junction anatomy in human teeth using scanning electron microscopy (SEM). Findings reveal Sharpey's fibers, junctional epithelium, and plaque distribution at the tooth surface.
Area of Science:
- Oral Biology
- Microscopy
- Dental Anatomy
Background:
- The dento-gingival junction is crucial for periodontal health.
- Understanding its microscopic structure is key to diagnosing and treating gingival diseases.
Purpose of the Study:
- To review the anatomical relationship of the dento-gingival junction in human dentition.
- To compare light microscopy and scanning electron microscopy (SEM) findings.
- To present novel SEM observations of the junctional tissues.
Main Methods:
- Extracted human teeth with marginal gingival tissue were used.
- Specimens were fixed, freeze-fractured, and processed using freeze-drying or critical point drying (CPD).
- Samples were coated with gold and examined using SEM.
Main Results:
- Numerous Sharpey's fibers were observed at the cemental surface.
- A dense mass of intermingled fibrils formed the tissue.
- Junctional epithelium and a homogeneous dental cuticle were identified.
- Plaque deposits extended to a cell-free zone on the tooth surface.
Conclusions:
- SEM provides detailed morphological insights into the dento-gingival junction.
- The study highlights the structural components and their arrangement.
- Comparison with light microscopy aids in understanding the junctional complex.