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Related Experiment Video

Updated: Feb 2, 2026

Polysome Purification from Soybean Symbiotic Nodules

Polysome Purification from Soybean Symbiotic Nodules

Published on: July 1, 2022

Callose-Regulated Symplastic Communication Coordinates Symbiotic Root Nodule Development.

Rocio Gaudioso-Pedraza¹, Martina Beck², Lisa Frances²

¹Centre for Plant Sciences, School of Biology, University of Leeds, Leeds LS2 9JT, UK.

Current Biology : CB

|November 13, 2018

Summary

This summary is machine-generated.

Rhizobia enhance symplastic communication in Medicago truncatula roots by reducing callose, facilitating nodule development. This process involves MtBG2, a callose-degrading enzyme, crucial for coordinating infection and development.

Keywords:

Medicago truncatula NODULE INCEPTION callose cell-to-cell communication legume root symbiosis nodule development rhizobial infection symbiotic gene expression symplastic connectivity β-1,3-glucanase

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MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

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Published on: March 5, 2014

Area of Science:

Plant Biology
Molecular Plant-Microbe Interactions
Cellular Communication

Background:

Legume nodule formation requires synchronized root responses for rhizobial infection.
Symplastic communication via plasmodesmata (PD) is critical for coordinating developmental processes.

Purpose of the Study:

To investigate the role of symplastic communication, regulated by callose turnover at PD, in Medicago truncatula nodule development.
To identify molecular mechanisms underlying rhizobia-induced symplastic changes.

Main Methods:

Spatiotemporal expression analysis of MtBG2.
In vivo transport assays using fluorescent molecules.
Genetic manipulation of callose levels and β-1,3-glucanase activity.
Analysis of nodulation phenotypes and key gene expression (NIN, NF-YA1).

Main Results:

Rhizobia reduce callose levels in nodule initiation sites, coinciding with MtBG2 expression.
MtBG2 degrades callose at PD, enhancing symplastic connectivity in infected tissues.
Modulating MtBG2 or callose levels significantly impacts nodule number and development.
Disrupted symplastic connectivity blocks NIN and NF-YA1 signaling.

Conclusions:

Symplastic intercellular communication, actively enhanced by rhizobia via MtBG2-mediated callose degradation, is essential for coordinating bacterial infection and nodule development in legumes.
MtBG2 plays a key role in establishing symplastic domains required for nodulation signaling pathways.