Related Experiment Video

Updated: Feb 2, 2026

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
10:09

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Published on: January 7, 2019

8.7K

The oncogenic role of MST3 in human gastric cancer

Kuo-Ting Lee1, Chia-Lin Chang2, Chung-Yen Li2

  • 1Department of Surgery, National Cheng Kung University Hospital Tainan, Taiwan, ROC.

American Journal of Cancer Research
|November 13, 2018
PubMed

Insights

Mammalian STE20-like kinase 3 (MST3) promotes gastric cancer growth and poor prognosis. Inhibiting MST3 suppressed tumor cell proliferation and growth, indicating its oncogenic role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Mammalian STE20-like kinase 3 (MST3) is a protein kinase involved in cell growth and apoptosis.
  • Previous research demonstrated MST3's role in promoting tumorigenicity in breast cancer via the VAV2/Rac1 signaling pathway.

Purpose of the Study:

  • To investigate the potential oncogenic role of MST3 in gastric cancer.
  • To determine the relationship between MST3 expression and patient prognosis.

Main Methods:

  • Immunohistochemistry was used to examine MST3 expression in 101 gastric cancer patient tissue samples.
  • Gastric cancer cell lines (MKN45 and NCI-N87) were transfected with MST3 shRNA to downregulate its expression.
  • In vitro assays included proliferation, anchorage-independent growth in soft agar, and in vivo tumor growth in NOD/SCID mice.

Main Results:

  • Higher MST3 expression was observed in gastric tumors compared to normal tissue.
  • High MST3 expression correlated with poor prognosis in gastric cancer patients.
  • Downregulation of MST3 inhibited gastric cancer cell proliferation, anchorage-independent growth, and tumor growth in mice.
  • MST3 shRNA enhanced p21 expression in MKN45 cells.

Conclusions:

  • MST3 plays a significant role in promoting gastric cancer progression.
  • MST3 expression levels can serve as a prognostic marker for gastric cancer patients.
  • Targeting MST3 may represent a potential therapeutic strategy for gastric cancer.
Keywords:
MST3anchorage-independent growthgastric cancerp21

More Related Videos

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue06:36

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Published on: May 1, 2019

7.4K
Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines08:42

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Published on: July 19, 2019

10.7K

Related Concept Videos

Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
11.5K
Role of Proteins in the Human Body01:28

Role of Proteins in the Human Body

Proteins are the building block of life. They are also  the most abundant macromolecules with as many diverse roles in the body. They are part of many structural components that provide unique shapes and structures to animal cells, tissues, and organs. In addition, they also act as biological catalysts and carry out several anabolic and catabolic reactions. Notably, some proteins are chemical messengers and regulate many critical processes, such as metabolism, growth, and development. They...
6.4K
Role of Water in Human Biology01:27

Role of Water in Human Biology

Water is the one of the most significant components of the human body; it plays a crucial role in several physiological activities because of its unique physicochemical properties. Importantly, it helps to regulate body temperature and is the chief component of several body fluids.
Water's Solvent Properties
Since water is a polar molecule with slightly positive and slightly negative charges, ions and polar molecules can readily dissolve in it. Therefore, it is referred to as a solvent, a...
13.5K
Gastric Motility01:16

Gastric Motility

Gastric motility is the coordinated contraction and relaxation of stomach muscles that convert ingested food into chyme, a semi-liquid substance ready for further digestion in the intestines. The process begins with the vagus nerve inducing the relaxation of the smooth muscles in the fundus and body of the stomach, allowing these regions to expand and accommodate up to approximately 1.5 liters of food and liquid.
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
3.2K
Gastric Emptying01:16

Gastric Emptying

Gastric emptying occurs when the stomach gradually releases chyme into the duodenum. When the stomach is distended, it triggers the release of gastrin, a hormone that promotes gastric acid secretion to aid in digestion. Additionally, stomach distension contributes to peristaltic waves that propel gastric contents toward the pyloric region. The gastroenteric reflex, on the other hand, primarily stimulates peristalsis in the intestines, facilitating the movement of contents further along the...
3.7K
Gastric Phase of Digestion01:26

Gastric Phase of Digestion

The gastric phase of digestion begins as soon as food enters the stomach. The incoming food bolus triggers neural and hormonal mechanisms, which last approximately 3 to 4 hours. During this phase, the stomach undergoes significant changes to prepare the food for further digestion and absorption.
When food enters the stomach, it stretches the stomach walls and activates stretch receptors. This triggers local reflexes of the enteric nervous system, mediated through the myenteric plexus. These...
3.4K