Size-selected Fe₃O₄-Au hybrid nanoparticles for improved magnetism-based theranostics

Size-selected Fe₃O₄-Au hybrid nanoparticles with diameters of 6-44 nm (Fe₃O₄) and 3-11 nm (Au) were prepared by high temperature, wet chemical synthesis. High-quality Fe₃O₄ nanocrystals with bulk-like magnetic behavior were obtained as confirmed by the presence of the Verwey transition. The 25 nm diameter Fe₃O₄-Au hybrid nanomaterial sample (in aqueous and agarose phantom systems) showed the best characteristics for application as contrast agents in magnetic resonance imaging and for local heating using magnetic particle hyperthermia. Due to the octahedral shape and the large saturation magnetization of the magnetite particles, we obtained an extraordinarily high r ₂-relaxivity of 495 mM^-1·s^-1 along with a specific loss power of 617 W·g_Fe ^-1 and 327 W·g_Fe ^-1 for hyperthermia in aqueous and agarose systems, respectively. The functional in vitro hyperthermia test for the 4T1 mouse breast cancer cell line demonstrated 80% and 100% cell death for immediate exposure and after precultivation of the cells for 6 h with 25 nm Fe₃O₄-Au hybrid nanomaterials, respectively. This confirms that the improved magnetic properties of the bifunctional particles present a next step in magnetic-particle-based theranostics.