Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.9K
Electronic Structure of Atoms02:28

Electronic Structure of Atoms

28.7K

An atom comprises protons and neutrons, which are contained inside the dense, central core called the nucleus, with electrons present around the nucleus. Taking into account the wave–particle duality of electrons and the uncertainty in position around the nucleus, quantum mechanics provides a more accurate model for the atomic structure. It describes atomic orbitals as the regions around the nucleus where electrons of discrete energy exist, characterized by four quantum...
28.7K
Correlations02:20

Correlations

35.9K
Correlation means that there is a relationship between two or more variables (such as ice cream consumption and crime), but this relationship does not necessarily imply cause and effect. When two variables are correlated, it simply means that as one variable changes, so does the other. We can measure correlation by calculating a statistic known as a correlation coefficient. A correlation coefficient is a number from -1 to +1 that indicates the strength and direction of the relationship between...
35.9K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

19.0K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
19.0K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

14.6K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
14.6K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vesicle3D: An Integrative Platform for 3D Segmentation and Analysis of Vesicles in Cryo-electron Tomograms.

Neuroscience bulletin·2026
Same author

SLC46A1 deficiency-mediated folate restriction suppresses colorectal cancer progression through epigenetic-transcriptional reprogramming.

Cell death & disease·2026
Same author

A distribution-aware semi-supervised pipeline for cost-effective neuron segmentation.

iScience·2026
Same author

Subcellular Structures in Native Hippocampal Synapses Revealed by Cryo-electron Tomography.

Neuroscience bulletin·2026
Same author

A Non-spinal Neural Circuit for Transmitting Information of Bladder Conditions.

Neuroscience bulletin·2025
Same author

[Retracted] MicroRNA-519a inhibits the proliferation and promotes the apoptosis of ovarian cancer cells through targeting signal transducer and activator of transcription 3.

Experimental and therapeutic medicine·2025
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: Feb 2, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.2K

Correlative light and electron microscopy for complex cellular structures on PDMS substrates with coded

Rong Sun1, Xi Chen, Chun-Ying Yin

  • 1Center for Integrative Imaging, National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.

Lab on a Chip
|November 13, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a new correlative light and electron microscopy (CLEM) system for precise ultrastructural analysis of neuronal synapses. The efficient technique combines fluorescence light microscopy (FLM) and electron microscopy (EM) for detailed cellular studies.

More Related Videos

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
09:18

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

81.8K
Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
10:00

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

Published on: June 5, 2011

15.9K

Related Experiment Videos

Last Updated: Feb 2, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

10.2K
Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
09:18

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

81.8K
Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
10:00

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy

Published on: June 5, 2011

15.9K

Area of Science:

  • Cell Biology
  • Neuroscience
  • Microscopy

Background:

  • Fluorescence light microscopy (FLM) offers specificity for cellular targets.
  • Electron microscopy (EM) provides high resolution for ultrastructural details.
  • Correlative light and electron microscopy (CLEM) integrates both techniques for enhanced biological insights.

Purpose of the Study:

  • To develop and validate an efficient and user-friendly CLEM system for studying neuronal synapses.
  • To enable precise co-registration of FLM and EM data for high-resolution analysis.
  • To investigate synaptic structures and synaptic vesicle docking in hippocampal neurons.

Main Methods:

  • Utilized micro-fabricated polydimethylsiloxane (PDMS) substrates with patterned symbols for coordinate encoding.
  • Integrated FLM for initial synapse localization with subsequent EM for ultrastructural analysis.
  • Developed custom software for automated EM image acquisition, FLM/EM alignment, and navigation.

Main Results:

  • Achieved high-quality electron tomograms of fluorescently labeled synapses in hippocampal neurons.
  • Enabled precise ultrastructural analysis of synaptic vesicle docking in different excitatory synapse subtypes.
  • Demonstrated efficient co-registration of FLM and EM images with high precision.

Conclusions:

  • The developed CLEM system offers an efficient approach for combining functional and ultrastructural analyses of neuronal synapses.
  • This technique is broadly applicable to the study of various subcellular structures requiring both specificity and resolution.
  • Facilitates detailed investigation into the heterogeneity of neuronal synapses.