miRNet-Functional Analysis and Visual Exploration of miRNA-Target Interactions in a Network Context
Yannan Fan1, Jianguo Xia2,3
1Institute of Parasitology, McGill University, Montreal, Quebec, Canada.
Abstract:
To gain functional insights into microRNAs (miRNAs), researchers usually look for pathways or biological processes that are overrepresented in their target genes. The interpretation is often complicated by the fact that a single miRNA can target many genes and multiple miRNAs can regulate a single gene. Here we introduce miRNet ( www.mirnet.ca ), an easy-to-use web-based tool designed for creation, customization, visual exploration and functional interpretation of miRNA-target interaction networks. By integrating multiple high-quality miRNA-target data sources and advanced statistical methods into a powerful network visualization system, miRNet allows researchers to easily navigate the complex landscape of miRNA-target interactions to obtain deep biological insights. This tutorial provides a step-by-step protocol on how to use miRNet to create miRNA-target networks for visual exploration and functional analysis from different types of data inputs.
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