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Published on: August 5, 2010
Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections
Hassan Valadbeigi1, Elham Esmaeeli1, Sobhan Ghafourian1
1Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Introduction:
The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam.
Materials And Methods:
For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes.
Results:
The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained.
Conclusion:
Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.
Insights
This study investigated virulence genes in uropathogenic Escherichia coli (UPEC) from urinary tract infections (UTIs). The fimH gene was most prevalent, indicating varied pathogenicity among UPEC strains.
Area of Science:
- Microbiology
- Infectious Diseases
- Genetics
Background:
- Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infections (UTIs).
- Understanding the prevalence of UPEC virulence genes is crucial for diagnosing and treating UTIs.
- Ilam province is a region where UPEC prevalence requires specific investigation.
Purpose of the Study:
- To determine the prevalence of key virulence genes in UPEC isolates from patients in Ilam.
- To analyze the distribution of virulence genes and their potential impact on pathogenicity.
Main Methods:
- A total of 80 UPEC isolates were collected from UTI patients over six months.
- Multiplex polymerase chain reaction (PCR) was employed to detect specific virulence genes: papEF, fimH, iucD, hlyA, fyuA, and ompT.
Main Results:
- The prevalence rates were: fimH (87.5%), ompT (71.2%), fyuA (67.5%), iucD (60%), papEF (47.5%), and hlyA (27.5%).
- The fimH gene showed the highest prevalence across the studied UPEC isolates.
- Twenty-seven distinct virulence gene profiles were identified among the isolates.
Conclusions:
- The fimH gene is the most common virulence factor in UPEC isolates from Ilam.
- The varied distribution of virulence genes suggests a diverse range of pathogenic potential among these UPEC strains.
- These findings contribute to understanding UPEC epidemiology and pathogenicity in the region.
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