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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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pre-mRNA Processing02:01

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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Chromatin Structure Regulates pre-mRNA Processing02:41

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Emission Spectra02:39

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When solids, liquids, or condensed gases are heated sufficiently, they radiate some of the excess energy as light. Photons produced in this manner have a range of energies, and thereby produce a continuous spectrum in which an unbroken series of wavelengths is present.
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MALDI-ToF MS Method for the Characterization of Synthetic Polymers with Varying Dispersity and End Groups
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Pre-Processing MALDI/TOF Mass Spectra by Using Geena 2.

P Romano1, A Profumo1, A Facchiano2

  • 1Ospedale Policlinico San Martino, Ospedale Policlinico San Martino, Genoa, Italy.

Current Protocols in Bioinformatics
|November 14, 2018
PubMed
Summary
This summary is machine-generated.

Geena 2 simplifies mass spectrometry data analysis by offering tools for filtering, averaging, and aligning spectra. This software supports scientists in handling large datasets for comparative studies.

Keywords:
MALDI/TOFmass spectrometrypre-processingspectra alignment

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Area of Science:

  • Mass Spectrometry
  • Bioinformatics
  • Data Analysis

Background:

  • High-throughput analysis of MALDI/TOF mass spectra generates large datasets.
  • Comparative studies require robust data processing and normalization.
  • Existing tools may lack comprehensive features for spectra manipulation.

Purpose of the Study:

  • To introduce Geena 2, a software tool for processing MALDI/TOF mass spectra.
  • To provide guidance on utilizing Geena 2's web interfaces for data analysis.
  • To offer protocols for data input and validation within Geena 2.

Main Methods:

  • Development of Geena 2 software with three distinct web interfaces.
  • Implementation of filtering, averaging, and aligning algorithms for mass spectra.
  • Creation of user support protocols for data input and file preparation.

Main Results:

  • Geena 2 provides a user-friendly platform for MALDI/TOF mass spectral data analysis.
  • The tool facilitates efficient handling of large-volume spectral data.
  • Web interfaces offer varying complexity levels to suit different user needs.

Conclusions:

  • Geena 2 is a valuable tool for scientists performing comparative mass spectrometry studies.
  • The software enhances data analysis efficiency and accessibility.
  • User support protocols ensure effective implementation for individual research.