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Updated: Feb 2, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Analysis of butterfly reproductive proteins using capillary electrophoresis and mass spectrometry
Maria Khihon Rokhas1, Johanna Liljestrand Rönn2, Christer Wiklund3
1KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Dept. of Chemistry, Div. of Applied Physical Chemistry, Analytical Chemistry, Stockholm, Sweden.
Abstract:
A method for analysis of proteins from spermatophores transferred from male to female Pieris napi butterflies during mating has been developed. The proteins were solubilized from the dissected spermatophores using different solubilization agents (water, methanol, acetonitrile and hexafluoroisopropanol). Capillary electrophoresis (CE) analysis was performed using an acidic background electrolyte containing a fluorosurfactant to avoid protein-wall adsorption, and to increase separation performance. The samples were also analyzed with matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), in a lower m/z range (1000-6000) and a higher m/z range (6000-12000). Solubilization with different solvents and the use of alternative matrices gave partly complementary profiles.
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