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Updated: Feb 2, 2026

Thermal Measurement Techniques in Analytical Microfluidic Devices
Published on: June 3, 2015
Adenosine Triphosphate Measurement in Deep Sea Using a Microfluidic Device
Tatsuhiro Fukuba1, Takuroh Noguchi2, Kei Okamura3
1Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima⁻cho, Yokosuka, Kanagawa 237-0061, Japan. bafuk@jamstec.go.jp.
Abstract:
Total ATP (adenosine triphosphate) concentration is a useful biochemical parameter for detecting microbial biomass or biogeochemical activity anomalies in the natural environment. In this study, we describe the development and evaluation of a new version of in situ ATP analyzer improved for the continuous and quantitative determination of ATP in submarine environments. We integrated a transparent microfluidic device containing a microchannel for cell lysis and a channel for the bioluminescence L⁻L (luciferin⁻luciferase) assay with a miniature pumping unit and a photometry module for the measurement of the bioluminescence intensity. A heater and a temperature sensor were also included in the system to maintain an optimal temperature for the L⁻L reaction. In this study, the analyzer was evaluated in deep sea environments, reaching a depth of 200 m using a remotely operated underwater vehicle. We show that the ATP analyzer successfully operated in the deep-sea environment and accurately quantified total ATP within the concentration lower than 5 × 10-11 M.
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