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Thermal Measurement Techniques in Analytical Microfluidic Devices
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Adenosine Triphosphate Measurement in Deep Sea Using a Microfluidic Device.

Tatsuhiro Fukuba1, Takuroh Noguchi2, Kei Okamura3

  • 1Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima⁻cho, Yokosuka, Kanagawa 237-0061, Japan. bafuk@jamstec.go.jp.

Micromachines
|November 15, 2018
PubMed
Summary

A new in situ adenosine triphosphate (ATP) analyzer was developed for continuous monitoring in deep-sea environments. This improved device accurately quantifies microbial activity and biomass at depths up to 200 meters.

Keywords:
ATPluciferin–luciferase assaymicrofluidic device

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Area of Science:

  • Biogeochemistry
  • Environmental Science
  • Biotechnology

Background:

  • Total adenosine triphosphate (ATP) concentration serves as a key biochemical indicator for microbial biomass and biogeochemical activity.
  • Detecting anomalies in these parameters is crucial for understanding natural environmental processes.
  • Existing methods for ATP analysis may have limitations in challenging environments like the deep sea.

Purpose of the Study:

  • To develop and evaluate an improved in situ ATP analyzer for continuous and quantitative determination of ATP in submarine environments.
  • To enhance the capability for real-time monitoring of microbial activity in deep-sea ecosystems.
  • To provide a robust tool for biogeochemical research in underwater settings.

Main Methods:

  • Integration of a transparent microfluidic device with microchannels for cell lysis and bioluminescence assay.
  • Incorporation of a miniature pumping unit and photometry module for bioluminescence intensity measurement.
  • Inclusion of a heater and temperature sensor to maintain optimal conditions for the luciferin-luciferase reaction.

Main Results:

  • Successful operation of the developed ATP analyzer in deep-sea environments up to 200 meters depth.
  • Accurate quantification of total adenosine triphosphate (ATP) with a lower detection limit of 5 × 10⁻¹¹ M.
  • Demonstration of the analyzer's capability for continuous and quantitative ATP determination in situ.

Conclusions:

  • The novel in situ ATP analyzer is effective for monitoring microbial biomass and activity in deep-sea environments.
  • The system provides accurate and continuous measurements, overcoming previous limitations.
  • This technology offers a valuable tool for advancing our understanding of submarine biogeochemical processes.