Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Expression of polymorphic B-cell antigens on human kidneys.

R J Hancock1, J Harvey, P R Evans

  • 1U.K. Transplant Service, Bristol, U.K.

Tissue Antigens
|April 1, 1988
PubMed
Summary

Researchers studied human kidney tissue using monoclonal antibodies. They found evidence of polymorphic class II determinants on kidneys, suggesting kidney-specific antigen expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibrillary glomerulonephritis and pulmonary haemorrhage in a Woolly monkey (Lagothrix lagotricha).

Journal of comparative pathology·2000
Same author

The role of early renal biopsy in cyclosporin induced thrombotic microangiopathy.

Pediatric nephrology (Berlin, Germany)·1999
Same author

Studies on the innervation of human renal allografts.

The Journal of pathology·1999
Same author

Ductal carcinomas of the prostate: a clinicopathological and immunohistochemical study.

British journal of urology·1998
Same author

Wegener's granulomatosis involving the urogenital tract.

British journal of urology·1996
Same author

Clinical features and outcome of patients with thin and ultrathin glomerular membranes.

QJM : monthly journal of the Association of Physicians·1995

Area of Science:

  • Immunology
  • Nephrology
  • Transplantation immunology

Background:

  • Polymorphic determinants of human leukocyte antigen (HLA) class II molecules are crucial in immune responses.
  • The expression and specificities of these determinants on human kidney tissues are not fully characterized.
  • Understanding kidney-specific antigen expression is vital for transplantation and autoimmune kidney diseases.

Purpose of the Study:

  • To investigate the expression of polymorphic B-cell determinants on human kidney tissues.
  • To determine if monoclonal antibodies against B-cell lines can identify specific determinants on kidney sections.
  • To explore the potential differences in antigen recognition between kidney tissue and lymphocytes.

Main Methods:

  • Analysis of 22 human kidney samples using mouse monoclonal antibodies.

Related Experiment Videos

  • Comparison of antibody reactivity patterns with kidney tissues and lymphocytes.
  • Serological analysis to assess antibody specificity for human leukocyte antigen (HLA) DR4.
  • Immunohistochemical staining of kidney sections.

    • Main Results:

    • Monoclonal antibodies reacted preferentially with kidneys from human leukocyte antigen (HLA) DR4 positive donors (p < 0.005).
    • The reactivity pattern on kidney tissues resembled that of antibodies to monomorphic class II determinants.
    • Antibodies did not exhibit clear DR4 specificity on lymphocytes in standard serological tests.
    • Evidence suggests the presence of polymorphic class II determinants on human kidneys.

    Conclusions:

    • Human kidneys express polymorphic class II determinants.
    • Differences in antibody specificity between kidney sections and lymphocytes warrant further investigation.
    • These findings contribute to understanding kidney immunobiology and potential transplantation compatibility markers.