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Related Experiment Video

Updated: Feb 2, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Portable Fluorescence Detection Platform with Integrating Sphere.

Dain M Harmon, Cheng-Fu Chen, John H Halford

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

|November 17, 2018

Summary

This summary is machine-generated.

A portable fluorescence detection platform was developed. This system shows a low limit of detection for fluorescein and DNA, with potential for even greater sensitivity.

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Last Updated: Feb 2, 2026

Fluorescence detection methods for microfluidic droplet platforms

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Published on: December 10, 2011

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Area of Science:

Analytical Chemistry
Biotechnology
Optical Engineering

Background:

Portable analytical devices are crucial for field-based measurements.
Fluorescence detection offers high sensitivity for various analytes.
Integrating spheres are effective for light collection in optical measurements.

Purpose of the Study:

To present a novel portable platform for fluorescence detection.
To evaluate the limit of detection for specific fluorescent analytes.
To assess the potential for improving the system's sensitivity.

Main Methods:

Development of a portable fluorescence detection platform integrated with an integrating sphere.
Testing the platform's performance using fluorescein solutions.
Quantifying the detection of dsDNA stained with SYBR-Green.

Main Results:

Demonstrated a lower limit of detection of 0.4 nM for fluorescein.
Achieved a lower limit of detection of 0.00128 ng/mL for SYBR-Green stained dsDNA.
Signal-to-noise ratio analysis indicated potential for further reduction in the limit of detection.

Conclusions:

The developed portable platform is effective for sensitive fluorescence detection.
The system shows promise for quantitative analysis of biomolecules like DNA.
Further optimization could enhance the sensitivity of this portable fluorescence detector.