Related Experiment Video
Updated: Feb 2, 2026
08:56
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
7.9K
Tandem Analysis by a Dual-Trap Miniature Mass Spectrometer
Xinwei Liu1, Xiao Wang2, Jiexun Bu2
1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument , Tsinghua University , Beijing 100084 , China.
Analytical Chemistry
|November 17, 2018
Summary
This study presents a novel miniature mass spectrometer with a dual-linear ion trap for in-situ analysis. It enables advanced tandem mass spectrometry techniques for precise qualitative and quantitative measurements of complex samples.
Area of Science:
- Analytical Chemistry
- Instrumentation Science
Background:
- Miniature mass spectrometers are gaining importance for on-site sample analysis.
- Coupling these devices with ambient ionization sources allows direct analysis of complex matrices.
Purpose of the Study:
- To develop a miniature mass spectrometer with a dual-linear ion trap (LIT) configuration.
- To enable comprehensive scan modes for high-quality qualitative and quantitative tandem mass spectrometry (MS/MS).
Main Methods:
- A discontinuous atmospheric pressure interface was employed for enhanced ion transfer.
- Real-time pressure control was integrated to optimize ion transfer and collision-induced dissociation (CID).
- Both beam-type CID and in-trap CID at high q were utilized for multi-stage fragmentation.
Main Results:
- The developed dual-LIT miniature mass spectrometer supports comprehensive scan modes for MS/MS.
- Real-time pressure control facilitated efficient ion transfer and CID.
- Demonstrated capabilities include multi-stage beam-type CID and in-trap CID for fragment ion accumulation.
- A precursor ion scan mode was successfully implemented for targeted analyte analysis.
Conclusions:
- The miniature mass spectrometer with a dual-LIT configuration offers advanced capabilities for in-situ analysis.
- The system supports high-quality qualitative and quantitative analyses through sophisticated MS/MS techniques.
- The demonstrated precursor ion scan mode enhances the analysis of specific target analytes.
More Related Videos
Related Concept Videos
01:16Mass Spectrometers
8.9K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
8.9K
01:21Tandem Mass Spectrometry
2.5K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.5K
01:33Peptide Identification Using Tandem Mass Spectrometry
8.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.4K
01:25IR Spectrometers
2.6K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
2.6K
01:41Social Traps
26.9K
Social traps are negative situations where people get caught in a direction or relationship that later proves to be unpleasant, with no easy way to back out of or avoid. The concept was orignally introduced by John Platt who applied psychology to Garrett Hardin's "Tragedy of the Commons", where in New England herd owners could let their cattle graze in the common ground. This situation seems like a good idea, but an individual could have an advantage. If they owned...
26.9K
01:21Mass Spectrometry: Complex Analysis
1.8K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.8K