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Interactions of oligochitosan with blood components
Tong Sun1, Xi Guo1, Rui Zhong2
1Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China; Neurosurgery Research Laboratory, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
International Journal of Biological Macromolecules
|November 17, 2018
Summary
Oligochitosan (OCHI) exhibits low red blood cell hemolysis and mild anticoagulation effects. Its high molecular weight fractions influence red blood cell aggregation and complement activation, with dose-dependent inhibition of C3a.
Area of Science:
- Biomaterials Science
- Biochemistry
- Hematology
Background:
- Oligochitosan (OCHI) possesses biological activities, but its interactions with blood components require clarification for biomedical applications.
- Understanding OCHI's structure-activity relationship is crucial for safe and effective use in medicine.
Purpose of the Study:
- To investigate the interactions of systematically prepared Oligochitosan (OCHI) with various blood components.
- To correlate these interactions with the molecular structures of OCHI, including molecular weight (MW).
Main Methods:
- OCHI preparation via hydrogen peroxide degradation and fractionation.
- Molecular structure characterization using GPC, FTIR, 1H, and 13C NMR.
- Assessment of OCHI interactions with red blood cells, coagulation, complement, and platelets.
Main Results:
- OCHI showed dose- and MW-dependent low hemolysis; high MW fractions affected RBC deformability and aggregation.
- OCHI demonstrated mild anticoagulation by inhibiting the intrinsic pathway, with identified corresponding MW.
- OCHI inhibited C3a dose-dependently and activated C5a via high MW fractions; no significant platelet effects were observed.
Conclusions:
- OCHI exhibits a generally favorable hemocompatibility profile with specific molecular weight-dependent effects on blood components.
- The study provides insights into OCHI's hemocompatibility mechanisms, guiding its future biomedical development.
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