Structural characteristics and anticancer/antioxidant activities of a novel polysaccharide from Trichoderma...

A novel water-soluble polysaccharide designated as TPS was isolated from the fermentation mycelia of Trichoderma kanganensis. TPS had a weight-average molecular mass of 3.074 × 10⁵ Da, and the monosaccharide composition was consisted of Man (45.5%), GlcA (5.5%), Glc (10%), and Gal (39%). The major backbone of TPS was →6-α-d-Galp-1→5-β-d-Manf-1→5,6-β-d-Manf-1→5,6-β-d-Manf-1→, and the side chains are α-d-Glcp-1→4-α-d-Glcp-1→, β-d-Galf-1→, and α-d-Glcp-1→. In addition, we demonstrated that TPS was non-toxic in normal cells (LO2 cells) and inhibited the proliferation of mouse colon cells (CT26 cells). TPS also showed free radical scavenging activity against hydrogen peroxide. Overall, these results suggested that TPS from Trichoderma kanganensis may have potential application in biomedical fields.