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Related Concept Videos

Body Temperature01:25

Body Temperature

4.4K
The body's temperature, measured in degrees, is determined by the balance between heat production and dissipation to the surrounding environment. For instance, if exercising vigorously, the body will produce more heat, causing sweat and dissipating that heat. Despite extreme environmental conditions and physical exertion, the human temperature-control system maintains a constant core body temperature (the temperature of deep tissues, which are the tissues located beneath the skin and other...
4.4K
Body Temperature01:07

Body Temperature

1.5K
Body temperature reflects the equilibrium between heat production and heat loss within the body. Most heat is generated by metabolically active tissues, particularly the liver, heart, brain, kidneys, and endocrine organs. At rest, skeletal muscles contribute 20–30% of total heat production, but during vigorous exercise, this can increase up to 30–40 times.
The average body temperature is approximately 37°C (98.6°F) and typically ranges from 36.1–37.2°C...
1.5K
Effects of Temperature on Free Energy02:11

Effects of Temperature on Free Energy

28.3K
The spontaneity of a process depends upon the temperature of the system. Phase transitions, for example, will proceed spontaneously in one direction or the other depending upon the temperature of the substance in question. Likewise, some chemical reactions can also exhibit temperature-dependent spontaneities. To illustrate this concept, the equation relating free energy change to the enthalpy and entropy changes for the process is considered:
28.3K
Factors Affecting Body Temperature01:28

Factors Affecting Body Temperature

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As a nurse, it is vital to understand the factors affecting body temperature to monitor variations and effectively evaluate deviations from regular.
Factors may  include:
9.0K
Increased Body Temperature01:25

Increased Body Temperature

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A body temperature above  38°C  (100.4 °F) is known as fever or pyrexia, and a person with fever is termed 'febrile.' Typically, the hypothalamus, a part of the brain that acts as the body's thermostat, regulates body temperature through a thermoregulatory setpoint. It receives signals from cold and warm thermal receptors throughout the body and adjusts the body's temperature accordingly. Fever occurs when this hypothalamic setpoint is altered, usually in...
7.5K
Decreased Body Temperature01:29

Decreased Body Temperature

1.1K
A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by...
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Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping
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Fluorescent nanodiamonds as a robust temperature sensor inside a single cell.

Takeharu Sekiguchi1, Shingo Sotoma1,2, Yoshie Harada1,3

  • 1Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.

Biophysics and Physicobiology
|November 20, 2018
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Fluorescent nanodiamonds (FNDs) offer robust, nanoscale temperature sensing within cells. This novel approach provides accurate, reliable cellular thermometry, unaffected by environmental factors.

Keywords:
cellular imagingfluorescent nanodiamondmagnetic resonancenitrogen-vacancy centertemperature sensing

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Cell Biology

Background:

  • Temperature is crucial for biological processes.
  • Accurate intracellular temperature measurement is challenging.
  • Fluorescent nanodiamonds (FNDs) are emerging as sensitive probes.

Purpose of the Study:

  • To evaluate FNDs as reliable nanoscale thermometers within single cells.
  • To assess the influence of cellular environmental factors on FND thermosensing.
  • To develop a simple protocol for absolute intracellular temperature measurement.

Main Methods:

  • Utilized FNDs with negatively-charged nitrogen-vacancy centers for fluorescence-based thermometry.
  • Investigated FND thermosensing under various environmental conditions (pH, ions, viscosity, etc.).
  • Developed and applied a protocol for single-cell temperature measurement using individual FNDs.

Main Results:

  • FND thermosensing ability demonstrated high robustness against environmental factors.
  • FNDs proved to be reliable thermometers in complex cellular environments.
  • Achieved absolute temperature measurement in single cells with accuracy better than ±1°C.

Conclusions:

  • FNDs are highly stable and reliable nanoscale thermometers for intracellular applications.
  • Their insensitivity to environmental variations ensures accurate biological temperature monitoring.
  • The developed protocol enables precise, single-cell thermometry crucial for biological studies.