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Purification of Rts1 RepA protein and binding of the protein to mini-Rts1 DNA
1Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
Journal of Bacteriology
|September 1, 1988
Abstract:
RepA protein, essential for the replication of plasmid Rts1, was purified, and its binding to mini-Rts1 subregions was examined by a DNase I protection assay. RepA protected the incI and incII iterons, a region immediately upstream of the repA promoter, and a 10-base-pair region located between the most external incII iteron and a GATC box. The protection was less efficient when preheated RepA was used.