Related Experiment Video
Updated: Feb 2, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Electron tomography in plant cell biology
Marisa S Otegui1,2,3, Jannice G Pennington4,5
1Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison WI, USA.
Abstract:
Electron tomography (ET) approaches are based on the imaging of a biological specimen at different tilt angles by transmission electron microscopy (TEM). ET can be applied to both plastic-embedded and frozen samples. Technological advancements in TEM, direct electron detection, automated image collection, and imaging processing algorithms allow for 2-7-nm scale axial resolution in tomographic reconstructions of cells and organelles. In this review, we discussed the application of ET in plant cell biology and new opportunities for imaging plant cells by cryo-ET and other 3D electron microscopy approaches.
More Related Videos
09:53Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
Published on: September 13, 2021
07:00Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Published on: August 5, 2021
Related Concept Videos
Plant Cell Wall
Plant Cell Wall
Plant Cells and Tissues
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Plant Hormones
Tonicity in Plants