Related Experiment Video
Updated: Feb 2, 2026

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
Published on: March 30, 2020
Low-cost compact multispectral spatial frequency domain imaging prototype for tissue characterization
Mohsen Erfanzadeh1, Sreyankar Nandy2, Patrick D Kumavor1
1Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Abstract:
We present a low-cost, compact, and multispectral spatial frequency domain imaging prototype. Illumination components, including 9 LEDs (660 nm - 950 nm) placed on a custom-designed printed circuit board, linear and rotational motors, a printed sinusoidal pattern, and collimation and projection optics as well as the detection components are incorporated in a compact custom-designed 3D-printed probe. Reconstruction of absorption and reduced scattering coefficients is evaluated via imaging tissue mimicking phantoms and potentials of the probe for biological tissue imaging are evaluated via imaging human ovarian tissue ex vivo.
Related Concept Videos
Linear Approximation in Frequency Domain
In contrast, nonlinear systems do not inherently possess these properties. However, for small deviations around an operating point, a nonlinear system can often be approximated as linear....
Frequency-Domain Interpretation of PD Control
The proportional control gain, combined with the...
Time and frequency -Domain Interpretation of PI Control
Acting as a low-pass filter, the PI controller slows the system's response and extends settling times. This requires...
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
Time and frequency -Domain Interpretation of Phase-lead Control
The design of phase-lead control involves the strategic placement of poles and zeros to balance steady-state error and system...
Time and frequency -Domain Interpretation of Phase-lag Control
Phase-lag controllers do not place a pole at zero, but instead influence the steady-state error by amplifying any...

