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Updated: Feb 2, 2026

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry

Published on: November 8, 2024

TILLING in Barley.

Matthias Jost¹, Miriam Szurman-Zubrzycka², Katarzyna Gajek²

¹Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Seeland, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|November 22, 2018

Summary

This summary is machine-generated.

Targeting Induced Local Lesions IN Genomes (TILLING) is a reverse genetics method for barley. This study details a chemical mutagenesis protocol and SNP detection methods for efficient gene function analysis in mutagenized barley populations.

Keywords:

LI-COR PCR SNP detection TILLING

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Last Updated: Feb 2, 2026

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry

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Published on: May 19, 2023

Area of Science:

Plant genetics
Molecular biology
Agricultural science

Background:

Targeting Induced Local Lesions IN Genomes (TILLING) is a key reverse genetics approach in barley research.
It facilitates the study of gene function by combining mutagenesis with mutation detection.
Existing TILLING populations in barley demonstrate the feasibility of this approach.

Purpose of the Study:

To describe a chemical mutagenesis protocol for barley seeds.
To present two independent procedures for efficient single nucleotide polymorphism (SNP) detection.
To enable functional analysis of genes in mutagenized barley populations.

Main Methods:

Chemical mutagenesis of barley seeds.
Single nucleotide polymorphism (SNP) detection using slab-gel-based electrophoresis on the LI-COR 4300 DNA Analyzer.
Single nucleotide polymorphism (SNP) detection using capillary gel-based electrophoresis on the AdvanCE FS96 instrument.

Main Results:

A detailed chemical mutagenesis protocol for barley is provided.
Two distinct and efficient SNP detection methods are described for large-scale analysis.
These methods are suitable for identifying variations in mutagenized barley plants.

Conclusions:

The described protocol enables the generation of TILLING populations in barley.
Efficient SNP detection methods facilitate the identification of gene variations.
This approach supports the functional characterization of barley genes.