Related Experiment Video
Updated: Feb 2, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping
Published on: January 8, 2008
Genotyping-by-Sequencing on the Ion Torrent Platform in Barley
Amina Abed1, Gaétan Légaré2, Sonia Pomerleau2
1Department de Phytologie and Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, QC, Canada.
Abstract:
The characterization of genetic polymorphism is a crucial step in both genetic studies and breeding programs. Genotyping-by-sequencing (GBS) constitutes one of the most attractive approaches for this purpose, especially in a genome as large as that of barley. The genome sequencing project undertaken by the International Barley Sequencing Consortium (IBSC) has produced a structured reference genome for the cultivar Morex [1] that can serve as an excellent resource for the analysis of GBS data. The genome assembly for this species [2] is thought to adequately capture the gene-rich portion of the genome (~80% of the entire genome). In this chapter, we describe the entire GBS process, from library preparation to the analysis of read data to produce a high-quality catalog of single nucleotide polymorphism (SNP) markers using the barley reference genome.
Related Concept Videos
Common Ion Effect
Precipitation of Ions
The equation that describes the equilibrium between solid calcium carbonate and its solvated ions is:
Ion Channels
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
Formation of Complex Ions
Ions and Ionic Charges
Ions as Acids and Bases
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:

