Bioinspired Photonic Barcodes with Graphene Oxide Encapsulation for Multiplexed MicroRNA Quantification

Area of Science:

• Biophotonics
• Nanotechnology
• Molecular Diagnostics

Background:

• Multiplexed microRNA (miRNA) quantification is crucial for clinical diagnosis.
• Existing methods face challenges in sensitivity, speed, and reproducibility for low-abundance targets.

Purpose of the Study:

• To develop novel mussel-inspired photonic crystal (PhC) barcodes with graphene oxide (GO) encapsulation for enhanced multiplexed miRNA detection.
• To improve the sensitivity, stability, and accuracy of miRNA quantification.

Main Methods:

• Immobilization of dispersed GO particles onto PhC barcodes using polydopamine adhesion.
• Decoration of PhC barcodes with GO to create a functional layer.
• Integration with a hybridization chain reaction amplification strategy for high-sensitivity detection.

Main Results:

• GO immobilization maintained PhC barcode microstructure, enhancing stability and reducing light scattering.
• High-sensitivity miRNA screening was achieved on GO-decorated PhC barcodes.
• Multiplexed quantification of low-abundance miRNAs was demonstrated rapidly, accurately, and reproducibly.

Conclusions:

• GO-encapsulated PhC barcodes offer a promising platform for advanced clinical diagnostics.
• The developed method enhances miRNA detection capabilities for practical applications.
• This technology facilitates accurate and efficient analysis of multiple miRNA targets.