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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

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High-resolution ex vacuo objective for cold atom experiments.

Xiao Li, Feng Zhou, Min Ke

|November 22, 2018

Summary

This summary is machine-generated.

We developed a cost-effective, versatile five-lens objective suitable for vacuum applications. It achieves high resolution and a wide field of view, adaptable to various wavelengths and window thicknesses for cold atom experiments.

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Area of Science:

Optical Engineering
Atomic Physics

Background:

Developing specialized optical objectives is crucial for advanced scientific research, particularly in fields like cold atom experiments.
Existing objectives may lack versatility or cost-efficiency for specific applications, such as those requiring vacuum compatibility.

Purpose of the Study:

To design and characterize a versatile, cost-efficient optical objective for use in vacuum environments.
To demonstrate the objective's adaptability to different experimental parameters like laser wavelengths and vacuum window thicknesses.

Main Methods:

A five-lens configuration using commercially available singlets was designed and constructed.
Optical performance, including numerical aperture (NA), resolution, and field of view, was simulated and experimentally verified.
Adaptability was tested by adjusting lens spacing for varying wavelengths and window thicknesses.

Main Results:

The home-built objective achieved a numerical aperture (NA) of 0.44 and a long working distance of 35.9 mm.
A diffraction-limited resolution of 1.08 μm and a field of view of approximately 210 μm were obtained with a 5 mm vacuum window at 780 nm.
The design demonstrated adaptability to laser wavelengths from 560-1000 nm and vacuum window thicknesses from 0-6 mm, maintaining NA > 0.43.

Conclusions:

The developed objective is versatile, cost-efficient, and suitable for ex vacuo utilization in scientific experiments.
Experimental results align well with simulations, confirming the objective's performance.
The objective has been successfully applied to single-atom trapping and detection, indicating broad potential in cold atom research.