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Foundations of immunohistochemistry. A practical review
1Department of Pathology and Laboratory Medicine, University of Minnesota School of Medicine, Minneapolis.
American Journal of Clinical Pathology
|September 1, 1988
Summary
This review details the historical development of enzyme-linked immunohistochemical methods for antigen localization. Horseradish peroxidase-linked procedures are foundational for modern diagnostic immunohistology and antigen detection.
Area of Science:
- Histopathology
- Immunochemistry
Background:
- Immunohistochemical methods have evolved significantly, leading to a complex array of reagents.
- Understanding the historical development is crucial for appreciating current techniques.
Purpose of the Study:
- To review the historic development of enzyme-linked immunohistochemical methods.
- To emphasize the foundational role of horseradish peroxidase-linked procedures in diagnostic immunohistology.
Main Methods:
- Review of historical enzyme-linked immunohistochemical procedures.
- Focus on antigen localization in routinely processed tissues.
- Emphasis on horseradish peroxidase-linked techniques.
Main Results:
- Enzyme-linked procedures have a rich history, with evolving technologies.
- Various antibodies, bridges, labels, and chromogens offer advantages and limitations.
- Horseradish peroxidase-linked procedures are central to contemporary diagnostic immunohistology.
Conclusions:
- The principles of horseradish peroxidase-linked procedures are fundamental to diagnostic immunohistology.
- These methods offer powerful antigen detection capabilities.
- They serve as a model for evaluating new immunohistochemical innovations.