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Published on: November 10, 2018
Detection of avian encephalomyelitis virus in chickens in Japan using RT-PCR
Yusuke Goto1, Gakuji Yaegashi1, Yoshihiro Kumagai1
1Iwate Prefecture Central Livestock Hygiene Service Center, 390-5 Sunakomi, Takizawa, Iwate 020-0605, Japan.
Abstract:
A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for broadly detecting the avian encephalomyelitis virus (AEV). The new primers were based on conserved sequences of the 5'-untranslated region of AEV, because the virus was not detected using previous reported RT-PCR. By applying this method to the chicken samples with suspected AEV infection in Japan, we successfully obtained PCR products of the predicted size from all samples, and we confirmed the presence of AEV via sequence analysis.
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