Related Experiment Video
Updated: Feb 2, 2026
16:49
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
Published on: July 16, 2012
12.0K
HCV-RNA Pcr Positivity In Hcv Antibody Negative Patients Undergoing Haemodialysis
Ismaa Ghazanfar Kiani1, Adil Naseer Khan2, Batool Butt1
1Military Hospital, Rawalpindi, Pakistan.
Journal of Ayub Medical College, Abbottabad : JAMC
|November 23, 2018
Summary
Hepatitis C virus (HCV) infection is common in haemodialysis patients. PCR testing is crucial for detecting HCV in serologically negative individuals, improving patient care.
Area of Science:
- Nephrology
- Hepatology
- Infectious Diseases
Background:
- Hepatitis C virus (HCV) affects 2.2% of the global population.
- HCV is a leading cause of chronic liver disease in haemodialysis patients.
- HCV can be present without detectable antibodies.
Purpose of the Study:
- To determine the frequency of HCV infection in haemodialysis patients.
- To identify HCV in patients who are serologically negative using PCR.
Main Methods:
- Cross-sectional study conducted from June to December 2016.
- Epidemiological data collected from 201 haemodialysis patients.
- Patients tested for Anti-HCV antibodies (ELISA) and HCV RNA (PCR).
Main Results:
- 17 out of 73 (23%) ELISA-negative patients were PCR-positive for HCV.
- The majority of PCR-positive patients were male (76.5%).
- Hypertension was the most common cause of chronic kidney disease (64.7%).
Conclusions:
- PCR detection of HCV-RNA is essential for accurate diagnosis in haemodialysis patients.
- Standard of care should include PCR for HCV detection in this population.
Related Concept Videos
00:58Positive, Negative, and Zero Work
22.2K
Work is done on an object when energy is transferred to the object. In other words, work is done when a force acts on a body that undergoes a displacement from one position to another. By definition, the work done by a force is the integral of the force with respect to the displacement along its path. Forces can vary as a function of position, and displacements can occur along various paths between two points. The magnitude of a force multiplied by the cosine of the angle that the force makes...
22.2K
01:18Positive and Negative Feedback Loops
25.2K
Animal organs and organ systems constantly adjust to internal and external changes through a process called homeostasis ("steady state"). Examples of these changes include regulation of the level of glucose or calcium in the blood or internal responses to external temperatures. Homeostasis requires maintaining an internal dynamic equilibrium:
25.2K
01:25Hemodialysis I: Introduction
1.7K
Hemodialysis (HD) is a medical treatment that artificially removes waste products, excess fluids, and toxins from the blood when the kidneys are no longer able to perform these functions effectively. In this process, blood is filtered through a semipermeable membrane, allowing for the selective removal of waste while preserving necessary components like blood cells and proteins. Hemodialysis is typically performed in patients with end-stage renal disease (ESRD) or severe kidney...
1.7K
01:24Hemodialysis II: Procedure and Complications
826
DialyzersA hemodialysis (HD) dialyzer is a plastic cartridge containing thousands of parallel hollow fibers, which serve as semipermeable membranes. These fibers are typically made from cellulose-based or other synthetic materials. During HD, blood is pumped into the top of the cartridge and distributed among these fibers. Simultaneously, dialysis fluid, known as dialysate, is introduced into the bottom of the cartridge, bathing the outside of the fibers. Across the semipermeable membrane,...
826
01:32PCR
237.4K
Overview
237.4K
02:32Position-effect Variegation
7.1K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
7.1K