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Published on: May 22, 2017
A Lysosome-Targetable Fluorescent Probe for Simultaneously Sensing Cys/Hcy, GSH, and H2S from Different Signal
Hui Zhang1,2, Lizhen Xu1, Wenqiang Chen1
1College of Chemistry and Materials Science, Guangxi Key Laboratory of Natural Polymer Chemistry and Physics , Guangxi Teachers Education University , 530001 Nanning , Guangxi , P. R. China.
Abstract:
Biothiols, a vital branch of reactive sulfur species (RSS) family, are indispensable in human physiology. However, the exact functional roles of each biothiol involved in complicated physiological activities are still not fully clarified. A critical barrier is a lack of robust molecular tools which can simultaneously visualize different biothiols with distinct emission signals. Herein, the first lysosome-targetable fluorescent probe, Lyso-RC, which could respond to Cys/Hcy, GSH, and H2S with different sets of signal patterns was developed. Lyso-RC responds to Cys/Hcy, GSH, and H2S with the fluorescence signal patterns of blue-red, green-red, and red, respectively. Significantly, Lyso-RC is capable of discriminating lysosomal Cys/Hcy, GSH, and H2S in HeLa cells.
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