Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

95.5K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
95.5K
Mitochondria01:37

Mitochondria

20.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
20.4K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

18.8K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

3.9K
3.9K
Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test01:22

Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test

200
In clinical practice, the direct measurement of hepatic blood flow to evaluate liver function presents significant challenges due to the intricate and specialized nature of the necessary techniques. Consequently, healthcare professionals often rely on empirical estimates derived from thorough patient examinations and liver function tests to gauge liver health. Among the tools at their disposal, the Child–Pugh and MELD scoring systems stand out for their ability to categorize and assess...
200
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

13.4K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial Calcium Transport in Amino Acid Metabolism: From nutritional responses to metabolic regulation.

American journal of physiology. Cell physiology·2026
Same author

Disruption of polycystin-1 cleavage impairs mitochondrial bioenergetics and calcium uptake in a substrate-dependent manner.

American journal of physiology. Renal physiology·2026
Same author

An open-source code to analyze mitochondrial intracellular distribution from fluorescence microscopy images.

Bioscience reports·2026
Same author

The past, present, and future of RNA biochemistry and mitochondrial research.

Trends in biochemical sciences·2026
Same author

GLP-1, Pancreatic β-Cells, and Insulin Secretion: What We Know and Where We Need to Go.

Diabetes·2026
Same author

Macrophages: their role in immunity and their relationship with fatty acids in health and disease.

Frontiers in immunology·2025

Related Experiment Video

Updated: Feb 2, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

40.6K

Fasting promotes functional changes in liver mitochondria.

Sergio L Menezes-Filho1, Ignacio Amigo1, Luis Alberto Luévano-Martínez2

  • 1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.

Biochimica Et Biophysica Acta. Bioenergetics
|November 23, 2018
PubMed
Summary
This summary is machine-generated.

Overnight fasting alters liver mitochondria function, reducing respiration and membrane potential. These findings suggest current isolation methods using fasted animals may need reconsideration.

Keywords:
CouplingFastingLiverMitochondriaPermeability transition

More Related Videos

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
08:54

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

Published on: April 27, 2015

17.4K
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
13:42

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Published on: September 7, 2012

22.1K

Related Experiment Videos

Last Updated: Feb 2, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

40.6K
Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
08:54

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

Published on: April 27, 2015

17.4K
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
13:42

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Published on: September 7, 2012

22.1K

Area of Science:

  • Mitochondrial physiology
  • Nutritional biochemistry

Background:

  • Rodent liver mitochondria isolation often involves overnight fasting.
  • The impact of fasting on mitochondrial function is not well understood.

Purpose of the Study:

  • To investigate the effects of overnight and short-term fasting on liver mitochondrial bioenergetic function.
  • To evaluate the physiological implications of fasting on mitochondrial parameters.

Main Methods:

  • Assessment of liver mitochondrial bioenergetic profile in fasted rodents.
  • Measurement of ADP-stimulated and maximal respiration.
  • Evaluation of mitochondrial membrane potential and calcium-induced permeability transition.

Main Results:

  • Overnight fasting (15h) significantly reduced ADP-stimulated and maximal respiration.
  • Fasting led to lower mitochondrial membrane potentials.
  • Reduced resistance to calcium-induced mitochondrial permeability transition was observed after fasting.
  • Short-term fasting (4h) induced similar changes, indicating a physiological response.

Conclusions:

  • Overnight fasting induces significant alterations in liver mitochondrial bioenergetics.
  • The common practice of using fasted animals for liver mitochondria isolation warrants re-evaluation.
  • Fasting-induced physiological changes in mitochondrial function are linked to nutritional status.