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Exploring anti-MRSA activity of chitosan-coated liposomal dicloxacillin

Aws Alshamsan1, Fadilah Sfouq Aleanizy1, Mohamed Badran1

  • 1Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Nanobiotechnology Unit, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Journal of Microbiological Methods
|November 23, 2018
PubMed

Insights

Chitosan-coated deformable liposomes effectively deliver dicloxacillin, enhancing its efficacy against antibiotic-resistant MRSA strains. This novel drug delivery system shows promise for treating challenging bacterial infections.

Area of Science:

  • Pharmaceutical Sciences
  • Nanotechnology
  • Infectious Diseases

Background:

  • Antibiotic resistance, particularly from methicillin-resistant Staphylococcus aureus (MRSA), poses a significant global health threat.
  • Existing antibiotics are becoming increasingly ineffective against resistant bacterial strains.
  • Novel drug delivery systems are crucial for enhancing the efficacy of existing antibiotics.

Purpose of the Study:

  • To evaluate the feasibility of chitosan-coated deformable liposomes (C-Lips) for delivering dicloxacillin (DLX).
  • To assess the efficacy of DLX-loaded liposomes (DLX-Lips) and C-Lips against MRSA.
  • To investigate the physicochemical properties and drug release kinetics of the developed liposomal formulations.

Main Methods:

  • DLX-loaded liposomes (DLX-Lips) were prepared using the lipid film hydration method.
  • Chitosan coating was applied to DLX-Lips via electrostatic deposition to form C-DLX-Lips.
  • Particle size, polydispersity index (PDI), zeta potential, drug entrapment efficiency (EE), and drug loading (DL) were characterized.
  • In vitro drug release and anti-MRSA activity were evaluated.

Main Results:

  • Both DLX-Lips and C-DLX-Lips exhibited nano-range particle size and narrow PDI.
  • Chitosan coating shifted zeta potential from negative to positive.
  • C-DLX-Lips showed significantly higher DLX entrapment efficiency (62%) and drug loading (5.6%) compared to DLX-Lips (38% and 3.1%).
  • C-DLX-Lips demonstrated a sustained in vitro drug release profile.
  • Both formulations displayed enhanced anti-MRSA activity.

Conclusions:

  • Chitosan-coated deformable liposomes are effective carriers for dicloxacillin.
  • These C-Lips enhance DLX efficacy against MRSA, offering potential for improved treatment of resistant infections.
  • The developed liposomal system holds considerable clinical value for the long-term use of DLX against MRSA.

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