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Production of Nanofibrillar Patterned Collagen for Tissue Engineering
Published on: September 20, 2024
977
Effect of functionalized gold nanoparticle on collagen stabilization for tissue engineering application.
Mohan Vedhanayagam1, Balachandran Unni Nair1, Kalarical Janardhanan Sreeram1
1CSIR-Central Leather Research Institute, Adyar, Chennai 600 020, India.
International Journal of Biological Macromolecules
|November 23, 2018
Summary
Functionalized gold nanoparticles maintain collagen’s triple helix structure. Dendrimer-functionalized nanoparticles show superior biocompatibility, making them promising for tissue engineering and cosmetics.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Biochemistry
Background:
- Collagen-based biomaterials require functionalization for stability and cell interaction.
- The impact of nanoparticle functionalization on collagen conformation is not well understood.
Purpose of the Study:
- To investigate how functionalized gold nanoparticles affect collagen's triple helical structure.
- To assess the biocompatibility of various functionalized gold nanoparticles with keratinocytes.
Main Methods:
- Gold nanoparticles (Au NPs) were synthesized and functionalized with organic molecules (SAM, APTES, pectin, chitosan, PAMAM dendrimer).
- Collagen conformational changes were analyzed using circular dichroism (CD) spectroscopy.
- Biocompatibility was evaluated using the MTT assay on HaCaT cells.
Main Results:
- Functionalized gold nanoparticles did not disrupt the triple helical conformation of collagen.
- Dendrimer-functionalized gold nanoparticles demonstrated higher keratinocyte viability compared to other functionalized nanoparticles.
- Collagen conformation was retained in the presence of functionalized gold nanoparticles.
Conclusions:
- Functionalized gold nanoparticles, particularly dendrimer-based ones, are suitable for collagen biomaterials.
- These materials show potential for applications in tissue engineering and cosmetic products.
- Dendrimer functionalization enhances the biocompatibility of gold nanoparticles with collagen.
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