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A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation
Published on: June 3, 2020
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Genome-wide association studies on the phyllosphere microbiome: Embracing complexity in host-microbe interactions
Kathleen Beilsmith1, Manus P M Thoen1, Benjamin Brachi2
1Department of Ecology and Evolution, University of Chicago, 1101 E 57th St, Chicago, IL, 60637, USA.
The Plant Journal : for Cell and Molecular Biology
|November 23, 2018
Summary
Genome-wide association studies (GWAS) can now identify plant genetic variations linked to complex phyllosphere microbiomes. This review explores early efforts and future directions for plant microbiome research using GWAS.
Area of Science:
- Plant biology
- Microbiome research
- Genetics
Background:
- Plants host diverse microbial communities that vary with environment.
- Previous methods for linking plant genetics to microbes were limited to simple, controlled interactions.
- Genome-wide association studies (GWAS) offer a powerful approach for studying plant-microbe interactions in complex communities.
Purpose of the Study:
- To review early applications of GWAS to the plant phyllosphere microbiome.
- To outline a workflow for conducting GWAS on phyllosphere microbiomes.
- To discuss how understanding plant-microbe interactions influences trait selection for GWAS.
Main Methods:
- Review of existing literature on GWAS for plant microbiomes.
- Adaptation of human microbiome GWAS workflows for plant studies.
- Consideration of ecological and evolutionary factors in trait mapping.
Main Results:
- GWAS is a promising tool for dissecting the genetic basis of plant-microbe associations.
- A structured workflow for phyllosphere microbiome GWAS can guide future research.
- Mechanisms, evolution, and environmental context are crucial for selecting relevant traits.
Conclusions:
- GWAS provides a robust framework for investigating the genetic underpinnings of plant microbiome composition.
- Future research should integrate ecological and evolutionary perspectives into GWAS of plant-microbe interactions.
- Standardized workflows will accelerate discoveries in plant microbiome genetics.
Keywords:
communityenvironmentgenome-wide association studiesgenotypehost-microbe interactionsmappingmicrobiomephenotypephyllospheresequencingMore Related Videos
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