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Progress in the 16S rRNA Gene Sequencing in Forensic Science.

G Q Song1,2,3, Y Cao2,3, H Li2,3

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Fa Yi Xue Za Zhi
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Forensic microbiology utilizes 16S rRNA gene sequencing for identifying biological samples, estimating time since death, and determining geographic origins. This method enhances traditional forensic techniques with high-throughput sequencing technology.

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RNA, ribosomal, 16Sforensic geneticsgenetics, microbialreview

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Area of Science:

  • Forensic Science
  • Microbiology
  • Genetics

Background:

  • Forensic microbiology is a key area in forensic science research.
  • The 16S rRNA gene is a conserved and specific marker ideal for forensic identification.
  • High-throughput sequencing technology enables advanced microbial research applicable to forensic science.

Purpose of the Study:

  • To describe research methods and sequencing technologies for 16S rRNA gene sequencing.
  • To summarize the research progress of 16S rRNA gene sequencing in forensic science.
  • To discuss the application value and future potential of 16S rRNA in forensic investigations.

Main Methods:

  • 16S rRNA gene sequencing techniques.
  • High-throughput sequencing technologies.
  • Analysis of microbial DNA for forensic applications.

Main Results:

  • 16S rRNA gene sequencing is applied to forensic practice.
  • Applications include biological sample identification, individual identification, postmortem interval estimation, and regional inference.
  • Forensic microbiology complements and assists traditional forensic methods.

Conclusions:

  • 16S rRNA gene sequencing offers significant value in forensic science.
  • Its application provides crucial clues for case investigations.
  • Continued research will expand its potential in forensic practice.