MiR-136 triggers apoptosis in human gastric cancer cells by targeting AEG-1 and BCL2

Related Experiment Video

Updated: Feb 2, 2026

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
08:42

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Published on: July 19, 2019

10.7K

L Yu1, G-Q Zhou, D-C Li

  • 1Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou City, Jiangsu Province, China. dechunli12@hotmail.com.

European Review for Medical and Pharmacological Sciences
|November 24, 2018
PubMed
Abstract

Insights

MicroRNA-136 (miR-136) is downregulated in gastric cancer. Overexpressing miR-136 promotes apoptosis by targeting AEG-1 and BCL2, suggesting miR-136 as a potential therapeutic target for gastric cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Gastric cancer is a leading cause of cancer-related deaths globally.
  • MicroRNAs are emerging as critical regulators of cancer development and potential therapeutic targets.
  • The role of miR-136 in gastric cancer pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the expression levels of miR-136 in gastric cancer.
  • To determine the therapeutic potential of miR-136 in gastric cancer treatment.
  • To identify the molecular targets of miR-136 in gastric cancer cells.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for transcript analysis.
  • Transfection of miR-136 mimics/plasmids using Lipofectamine 2000.
  • Apoptosis assays (DAPI, acridine orange/ethidium bromide staining) and Western blotting for protein expression analysis.

Main Results:

  • miR-136 expression was significantly downregulated in gastric cancer cells.
  • Overexpression of miR-136 promoted apoptosis in gastric cancer cells.
  • miR-136 directly targeted and downregulated AEG-1 and BCL2 protein expression.

Conclusions:

  • miR-136 induces apoptosis in gastric cancer cells through targeting AEG-1 and BCL2.
  • Restoring miR-136 levels represents a promising therapeutic strategy for gastric cancer.
  • Further research into miR-136-based therapies for gastric cancer is warranted.

More Related Videos

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue06:36

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Published on: May 1, 2019

7.4K
Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal11:21

Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal

Published on: January 26, 2019

10.4K

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.9K
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
14.5K
Gastric Motility01:16

Gastric Motility

Gastric motility is the coordinated contraction and relaxation of stomach muscles that convert ingested food into chyme, a semi-liquid substance ready for further digestion in the intestines. The process begins with the vagus nerve inducing the relaxation of the smooth muscles in the fundus and body of the stomach, allowing these regions to expand and accommodate up to approximately 1.5 liters of food and liquid.
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
3.2K
Gastric Emptying01:16

Gastric Emptying

Gastric emptying occurs when the stomach gradually releases chyme into the duodenum. When the stomach is distended, it triggers the release of gastrin, a hormone that promotes gastric acid secretion to aid in digestion. Additionally, stomach distension contributes to peristaltic waves that propel gastric contents toward the pyloric region. The gastroenteric reflex, on the other hand, primarily stimulates peristalsis in the intestines, facilitating the movement of contents further along the...
3.7K
Gastric Phase of Digestion01:26

Gastric Phase of Digestion

The gastric phase of digestion begins as soon as food enters the stomach. The incoming food bolus triggers neural and hormonal mechanisms, which last approximately 3 to 4 hours. During this phase, the stomach undergoes significant changes to prepare the food for further digestion and absorption.
When food enters the stomach, it stretches the stomach walls and activates stretch receptors. This triggers local reflexes of the enteric nervous system, mediated through the myenteric plexus. These...
3.4K
Target Cell Response to Hormones01:22

Target Cell Response to Hormones

Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
5.7K