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Fisetin protects against rotenone-induced neurotoxicity through signaling pathway

Malathi Rajendran1, Rajamurugan Ramachandran1

  • 1Department of Biochemistry, Research and Development Centre, Bharathiar University, Coimbatore- 641046, Tamil Nadu, India.

Frontiers in Bioscience (Elite Edition)
|November 24, 2018
PubMed

Insights

Fisetin protects against rotenone-induced neurotoxicity in Parkinson

Area of Science:

  • Neuroscience
  • Cell Biology
  • Pharmacology

Background:

  • Parkinson's disease (PD) is a neurodegenerative disorder.
  • Rotenone exposure models PD-like neurotoxicity.
  • SH-SY5Y neuroblastoma cells are a common cellular model for PD research.

Purpose of the Study:

  • To evaluate the protective effect of fisetin against rotenone-induced toxicity.
  • To investigate the molecular mechanisms underlying fisetin's neuroprotection.

Main Methods:

  • SH-SY5Y cells were pretreated with fisetin before rotenone exposure.
  • Cell viability, oxidative stress, and apoptosis markers (caspase-3, Bax, Bcl-2) were assessed.
  • Key signaling pathways (p38/JNK-MAPK, PI3K/Akt/GSK-3β) were analyzed.

Main Results:

  • Rotenone significantly decreased cell viability, increased oxidative stress, and induced apoptosis.
  • Fisetin pretreatment dose-dependently alleviated rotenone-induced cytotoxicity and oxidative stress.
  • Fisetin modulated apoptosis-related proteins (Bax, Bcl-2) and key signaling pathways.

Conclusions:

  • Fisetin exhibits significant neuroprotective effects against rotenone-induced toxicity in SH-SY5Y cells.
  • Fisetin's protective mechanisms involve the modulation of apoptosis and multiple signaling pathways.
  • Fisetin shows potential as a therapeutic agent for Parkinson's disease.

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