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Updated: Feb 2, 2026

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
Published on: October 13, 2015
Rapid detection of beta-lactamase production including carbapenemase by thin layer chromatography
Zeynep Kanlidere1, Onur Karatuna2, Tanil Kocagöz3
1Üsküdar University, Faculty of Engineering and Natural Sciences, Department of Chemical and Biological Engineering, Istanbul, Turkey; Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Medical Biotechnology, Istanbul, Turkey.
Objectives:
To develop a rapid and simple method that can identify the presence of β-lactamases in clinical isolates and samples, and determine their activity on different types of β-lactam antibiotics, including carbapenems, within one hour.
Methods:
In this study, we describe a thin layer chromatography-based method for rapid detection of β-lactamases including carbapenemases. The method relies on the examination of changes in the migration rate of β-lactams in chromatography, due to degradation by β-lactamase enzymes. A total of 44 isolates, 29 carbapenemase-producers and 15 non-carbapenemase-producers, were screened by this method.
Results:
The method has proven to be able to distinguish β-lactamases as carbapenemase or non-carbapenemase producing strains with high sensitivity in one hour.
Conclusions:
The method developed, provides information about the production of β-lactamases by bacteria and β-lactam drugs inactivated by these enzymes, including carbapenems. This new method may play an important role in guiding antimicrobial treatment, especially in critically ill patients infected bacteria producing β-lactamases.
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