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    Area of Science:

    • Biomedical Optics
    • Medical Imaging

    Background:

    • Fiber-based polarization-sensitive optical coherence tomography (PS-OCT) offers intrinsic contrast for birefringent tissues in luminal organs.
    • Extracting tissue optic axis (OA) orientation enhances PS-OCT's diagnostic potential.
    • Existing methods suffer from measurement distortions, hindering accurate OA determination.

    Purpose of the Study:

    • To present a straightforward calibration technique for recovering accurate sample OA measurements in endoscopic PS-OCT.
    • To enable reliable orientation information extraction from birefringent tissues.

    Main Methods:

    • Development of a calibration technique for PS-OCT systems.
    • Implementation without requiring hardware modifications to existing systems.
    • Application to fiber-based, rotationally-scanning endoscopic catheters.

    Main Results:

    • Successful recovery of sample OA measurements previously unobtainable due to distortion.
    • Demonstration of a generally applicable calibration method.
    • Validation of the technique's potential for improving endoscopic PS-OCT data utility.

    Conclusions:

    • The presented calibration technique significantly enhances the accuracy of OA measurements in PS-OCT.
    • This hardware-independent method is broadly applicable, improving endoscopic imaging of luminal organs.
    • The technique holds substantial promise for advancing biomedical applications of PS-OCT.