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Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

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Published on: June 13, 2018

Light-sheet based two-dimensional Scheimpflug lidar system for profile measurements.

Fei Gao, Hongze Lin, Kun Chen

|November 25, 2018

Summary

This study introduces a novel 2D Scheimpflug lidar system for precise 3D surface profiling. The developed light-sheet lidar achieves accurate measurements with minimal error, showing great potential for various applications.

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Area of Science:

Optics and Photonics
Metrology
Computer Vision

Background:

Traditional 3D surface measurement techniques often face limitations in accuracy, speed, or complexity.
The Scheimpflug principle offers a geometric solution for imaging at an angle, crucial for lidar systems.

Purpose of the Study:

To develop and validate a novel 2D Scheimpflug lidar system for high-resolution 3D surface profiling.
To investigate the theoretical underpinnings and practical implementation of a light-sheet based Scheimpflug lidar.

Main Methods:

Development of a light-sheet based 2D Scheimpflug lidar system.
Theoretical analysis of the geometrical relationships governing the lidar system's operation.
Experimental validation using diverse objects like a plastic bowl, carton box, and manikin.

Main Results:

Successful realization of a 2D Scheimpflug lidar system capable of 3D surface measurements.
Reconstructed image dimensions closely matched physical objects, with errors around the millimeter range at close distances.
Demonstrated feasibility of 3D profile measurements for various geometries.

Conclusions:

The developed 2D Scheimpflug lidar system is effective for 3D surface profiling.
The system exhibits high accuracy and significant potential for close-range metrology applications.
This novel approach offers a promising alternative for non-contact 3D surface characterization.