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Distance Corrections01:15

Distance Corrections

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To achieve precise distance measurements, especially in surveying and construction, certain corrections must be applied to account for potential sources of error like the standardization errors, temperature variations, and slope adjustments.Standardization error emerges when measurement equipment undergoes changes, such as wear, repairs, or weather impacts. To address this, surveyors compare the equipment’s readings to a standard. This process identifies any deviation that might lead to...
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Power Factor Correction01:20

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The power transmission to a factory involves the transfer of apparent power, a combination of active and reactive power. The power factor measures how effectively electrical power is converted into useful work output. The ratio of the real power (KW) that does the work to the apparent power (KVA) supplied to the circuit.
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Endoscopic Procedures III: Video Capsule Endoscopy01:28

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NMR Spectrometers: Resolution and Error Correction01:14

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When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
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In-situ Hybridization02:31

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Related Experiment Video

Updated: Feb 2, 2026

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
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Published on: January 25, 2008

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Two-photon lensless micro-endoscopy with in-situ wavefront correction.

Uri Weiss, Ori Katz

    Optics Express
    |November 25, 2018
    PubMed
    Summary

    Researchers developed a new method for in-situ wavefront correction in multi-core fiber endoscopes, enabling deep tissue imaging without needing distal access. This breakthrough advances minimally-invasive microscopy techniques.

    Area of Science:

    • Biomedical Optics
    • Microscopy
    • Fiber Optics

    Background:

    • Multi-core fiber-bundle endoscopes offer minimally-invasive deep tissue imaging and opto-genetic stimulation.
    • Wavefront-shaping corrects distortions in fiber endoscopes, enabling lensless micro-endoscopy.
    • Current methods require distal access to the fiber end for wavefront correction.

    Purpose of the Study:

    • To develop an in-situ method for determining wavefront correction in multi-core fiber endoscopes without distal access.
    • To enable lensless, deep-tissue, three-dimensional imaging using adaptive optics in fiber bundles.

    Main Methods:

    • Exploiting the nonlinearity of two-photon excited fluorescence.
    • Adaptively determining wavefront correction using only proximal detection of epi-detected fluorescence.

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  • Utilizing commercially-available ordered- and disordered multi-core fiber bundles.
  • Main Results:

    • Successfully demonstrated in-situ wavefront correction without distal access.
    • Achieved diffraction-limited, three-dimensional, two-photon lensless microendoscopy.
    • Validated the method with both ordered and disordered fiber bundles.

    Conclusions:

    • The developed method enables in-situ wavefront correction for multi-core fiber endoscopes.
    • This technique overcomes the limitation of requiring distal access, simplifying deep tissue imaging.
    • The findings pave the way for advanced minimally-invasive endoscopic imaging and opto-genetic applications.