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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
Published on: September 15, 2017
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Multi-dimensional in vitro bioactivity profiling for grouping of glycol ethers
Fabian A Grimm1, John S House2, Melinda R Wilson1
1Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, 77843, USA.
Regulatory Toxicology and Pharmacology : RTP
|November 25, 2018
Summary
This study explored chemical grouping for safety assessments using in vitro models. Glycol ether bioactivity in vitro correlated with alcohol chain length and in vivo irritation, aiding chemical safety evaluations.
Area of Science:
- Toxicology
- In Vitro Assays
- Chemical Safety Assessment
Background:
- Read-across for chemical safety relies on grouping similar substances.
- Grouping challenges exist for chemicals with limited bioactivity data.
- In vitro models offer physiologically relevant data for improved read-across.
Purpose of the Study:
- To assess in vitro bioactivity profiles of glycol ethers for data-derived chemical groupings.
- To explore grouping strategies for chemicals with low bioactivity.
- To correlate in vitro findings with in vivo toxicity and irritation data.
Main Methods:
- Comprehensive bioactivity profiling using organotypic and population-based in vitro models.
- Cytotoxicity assays in induced pluripotent stem cell-derived hepatocytes and cardiomyocytes.
- Phenotypic and transcriptomic assays, cytotoxicity in human lymphoblast cell lines, and Toxicological Priority Index (ToxPi) analysis.
Main Results:
- Glycol ethers showed cytotoxicity only at high concentrations in hepatocytes and cardiomyocytes.
- Inter-individual variability in cytotoxicity was observed across human lymphoblast cell lines, but no population-specific effects.
- In vitro bioactivity trends were consistent between cell types, suggesting a universal mode of action.
- Glycol ethers grouped by alcohol chain length, with longer chains showing increased bioactivity.
- In vitro bioactivity correlated with in vivo irritation scores for E-series glycol ethers.
Conclusions:
- In vitro bioactivity profiling can support data-derived groupings for glycol ethers.
- Alcohol chain length is a key factor in glycol ether in vitro bioactivity.
- In vitro data, particularly for E-series glycol ethers, shows potential for predicting in vivo irritation.
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