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Updated: Feb 2, 2026

Bottlenose Dolphin Tursiops truncatus Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization
Published on: August 21, 2017
Post-thawing effects of three cryopreservation diluents on Rusa deer (Rusa timorensis) spermatozoa
Davide Monaco1, Eduardo Zappia1,2, Sudsukh Apichaya3,4
1Department of Veterinary Medicine (Di.Me.V.), University of Bari Aldo Moro, Valenzano, Italy.
Abstract:
The aim of this study was to evaluate home-made and commercial extenders for the cryopreservation of Rusa deer semen. After collection by electroejaculation, six ejaculates were diluted and frozen in TES-based, Tris-based and Triladyl® extenders. Subjective motility, viability, morphology, acrosome integrity and membrane functionality were assessed post-thawing and after 1-hr incubation at 37°C (Thermal stress test). Total and progressive motility, and kinematic parameters were also assessed through CASA system. Post-thawing sperm progressive motility (PM), velocity according to the straight path (VSL) and linearity (LIN) showed significant differences, and higher values were detected for spermatozoa diluted with Triladyl® and TES (p < 0.05) as compared with Tris (PM of Triladyl® 14.7% vs. 3.2% TES and 2.5% Tris; VSL 56 for Triladyl® , 59.2 for TES and 41.7 for Tris; LIN 45.6 for Triladyl® , 52 for TES and 36.5 for Tris). Triladyl® and TES extender led to better post-thawing sperm parameters, but these preliminary results need to be verified through artificial insemination trials.
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